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PCR products of 164 mCysLT1 ; , 182 mCysLT2 ; , and 211 bp G3PDH ; . The standards and the samples were simultaneously amplified using the same reaction master mixture. The reactions were incubated at 95 C for 10 min to activate the polymerase, followed by 50 cycles of amplification. Each cycle of PCR included 3 sec of denaturation at 95 C, 3 sec of primer annealing at 67 C for G3PDH, 65 C for mCysLT1, and 68 C for mCysLT2, and 10 sec of extension at 72 C. The temperature ramp was 20 C sec. At the end of the extension steps fluorescence of each sample was measured to allow quantification of the cDNAs. After cycling, melting curves of the PCR products were acquired by stepwise increase of the temperature from 5 C above the annealing temperature to 95 C. Using the LightCycler analysis software, the SYBR Green I signal of each sample was plotted versus the number of cycles and the crossing points were obtained. These crossing points correlate inversely with and micafungin.
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Payment must be made in US dollars. Payment may be made by check or travelers check. VISA, MasterCard, or American Express will be charged in US dollars. Signature, account number, and expiration date must be included. Non-US checks written in US$ must be written on banks with a US counterpart. Phone charges will NOT be accepted. If payment is being made by your company, please make sure your name is indicated on the check stub or correspondence. If ISPOR cannot verify your current membership, you will be charged the non-member registration rate. * One Day Registration does not include membership benefits. When you register as a non-member, you receive ISPOR membership and a one-year, hard copy subscription to VALUE IN HEALTH-The Journal of the International Society for Pharmacoeconomics and Outcomes Research. Cancellation fee before May 1, 2002 is US 0. No refunds given after May 1, 2002.
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