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Lactose intolerance. Some people are born with this and others acquire it during life or after a gastro-intestinal infection. Drinking too much cow's milk and its products like cheese and yoghurt can cause bloating and diarrhoea. Try a lactose free period to see if it helps, soya milk is fine. If symptoms are better on a lactose free diet, add milk back in to the diet to determine how much you can tolerate. If you stay off diary products you may need calcium supplements. Gluten withdrawal. Gluten is a protein in wheat, barley and rye, in patients with Coeliac disease it is toxic to the small bowel and causes bloating, diarrhoea, weight loss or anaemia. Treatment is life long avoidance of gluten in food. In some patients with IBS a gluten free diet can help also but this does not need to be life long and can be guided by symptoms. The main sources of gluten are anything made from wheat flour such as breads, cakes, biscuits, pasta. The following list is intended as a general guide only. Note: In this table, "check" indicates that some brands contain gluten, others are gluten-free. Ingredient lists of all food products should be checked. Foods to Avoid Flour Wheat flour, rye flour, barley flour, wheaten cornflour, triticale flour Bread All bread including wheat, rye and sourdough bread, biscuits, pastries, buns, muffins, pikelets, crumpets, croissants, breadcrumbs unless labelled gluten-free ; Cereals Breakfast cereals containing wheat, oats * , semolina, barley, rye, malt extract, wheatbran, oatbran * Pasta and grains Wheaten noodles, pasta, spaghetti, vermicelli & instant pasta meals. Fruit Commercial thickened fruit pie filling Vegetables Canned or frozen vegetables in sauce, commercially prepared vegetable and potato salad unless dressing checked ; . Meat, Fish & Poultry Foods prepared or thickened with flour, batter or crumbs, sausages, most processed meats and fish, corned beef, meat pies, frozen dinners Foods to Include Rice flour, pure maize cornflour, cornmeal polenta, soya flour, potato flour, arrowroot, buckwheat, sorghum, millet, sago, tapioca, lentil flour, baby rice cereal, amaranth, lupin Rice cakes, corn cakes, some rice crackers check gluten-free bread, biscuits, pastries, rolls, breadcrumbs, cakes, and desserts made from allowed flours; gluten-free bread, biscuit, cake mixes. Rice, corn and soy breakfast cereals check ; , glutenfree muesli, homemade muesli using allowed ingredients Rice, corn, cornmeal, tapioca, buckwheat and glutenfree pastas, rice noodles, rice vermicelli.Rice, buckwheat, polenta Fresh, frozen, canned or dried fruit, fruit juices Fresh, frozen, dehydrated, or canned vegetables without sauces, vegetable juices. Fresh, smoked, cured, frozen without sauces, crumbs or batters. Canned meat or fish without sauce or cereal. Ham off the bone check ; Bacon, gluten-free sausages
Tom, you needn't get me the horses. I don't want to go, " she said. "Why not, Miss Eva?" "These things sink into my heart, Tom, " said Eva, --"they sink into my heart, " she repeated, earnestly. "I don't want to go; " and she turned from Tom, and went into the house. A few days after, another woman came, in old Prue's place, to bring the rusks; Miss Ophelia was in the kitchen. "Lor!" said Dinah, "what's got Prue?" "Prue isn't coming any more, " said the woman, mysteriously. "Why not?" said Dinah. "she an't dead, is she?" "We doesn't exactly know. She's down cellar, " said the woman, glancing at Miss Ophelia. After Miss Ophelia had taken the rusks, Dinah followed the woman to the door. "What has got Prue, any how?" she said. The woman seemed desirous, yet reluctant, to speak, and answered, in low, mysterious tone. "Well, you mustn't tell nobody, Prue, she got drunk agin, --and they had her down cellar, --and thar they left her all day, --and I hearn 'em saying that the flies had got to her, --and she's dead!" Dinah held up her hands, and, turning, saw close by her side the spirit-like form of Evangeline, her large, mystic eyes dilated with.
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Protein, and that of the pstA1-1 cells is 0.46 mol mg of protein, which reflects the differences in buoyant densities. These data are entirely consistent with the pstA1 strain having a defect in lipid traffic to the mitochondria. The data also clearly demonstrate that mitochondrial populations of at least two different densities can be found in both the parental and mutant cells. Mitochondria from pstA1-1 Cells Have a Minor Defect in Protein Import--Since our experimental results demonstrated defects in both lipid traffic to the mitochondria and abnormal organelle density, we also examined the import of proteins into purified mitochondria prepared on Nycodenz gradients 25 ; . For these studies, we employed an Su9-DHFR chimeric construct consisting of 69 amino acids from the N terminus of subunit 9 of the mitochondrial ATPase and mouse dihydrofolate reductase 32 ; that is routinely used to study the import of matrix proteins. In addition, we measured the import and processing of Psd1p. The [35S]methionine-labeled precursor proteins were produced using a coupled transcription translation reaction 30 ; , and the time- and membrane potential ; dependent import of the proteins was measured as shown in Fig. 9. The precursor for Su9-DHFR is readily imported into the mitochondria and processed to its mature form in both parental and pstA1-1 cells. The rate of Su9-DHFR import into mutant mitochondria is 60% of that found for parental mitochondria. The precursor for Psd1p is also imported into the mitochondria and targeted to the inner membrane of both parental and mutant strains. Consistent with stepwise processing of the mitochondrial targeting sequence and the inner membrane sorting sequence, two precursor forms of Psd1p designated pI and pII ; are identifiable. The data in Fig. 9 show and mechlorethamine
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