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65. Bartoloni A, Guglielmetti P, Cancrini G, et al. Comparative efficacy of a single 400 mg dose of albendazole or mebendazole in the treatment of nematode infections in children. Trop Geogr Med. 1993; 45 3 ; : 114-116. 66. Sinniah B, Sinniah D. The anthelmintic effects of pyrantel pamoate, oxantel-pyrantel pamoate, levamisole and mebendazole in the treatment of intestinal nematodes. Ann Trop Med Parasitol. 1981; 75 3 ; : 315-321. 67. Lucas AO, Oduntan SO. Treatment of hookworm infection and other intestinal parasites with 1-tetramisole Ketrax ; . Ann Trop Med Parasitol. 1972; 66 3 ; : 391-398. 68. Charoenlarp P, Waikagul J, Muennoo C, et al. Efficacy of single-dose mebendazole, polymorphic forms A and C, in the treatment of hookworm and Trichuris infections. Southeast Asian J Trop Med Public Health. 1993; 24 4 ; : 712-716. 69. Legesse M, Erko B, Medhin G. Comparative efficacy of albendazole and three brands of mebendazole in the treatment of ascariasis and trichuriasis. East Afr Med J. 2004; 81 3 ; : 134-138. 70. Juan JO, Lopez Chegne N, Gargala G, Favennec L. Comparative clinical studies of nitazoxanide, albendazole and praziquantel in the treatment of ascariasis, trichuriasis and hymenolepiasis in children from Peru. Trans R Soc Trop Med Hyg. 2002; 96 2 ; : 193-196. 71. Hall A, Nahar Q. Albendazole and infections with Ascaris lumbricoides and Trichuris trichiura in children in Bangladesh. Trans R Soc Trop Med Hyg. 1994; 88 1 ; : 110-112. 72. Belizario VY, Amarillo ME, de Leon WU, et al. A comparison of the efficacy of single doses of albendazole, ivermectin, and diethylcarbamazine alone or in combinations against Ascaris and Trichuris spp. Bull World Health Organ. 2003; 81 1 ; : 35-42. 73. Stephenson LS, Kinoti SN, Latham MC, Kurz KM, Kyobe J. Single dose metrifonate or praziquantel treatment in Kenyan children, I: effects on Schistosoma haematobium, hookworm, hemoglobin levels, splenomegaly, and hepatomegaly. J Trop Med Hyg. 1989; 41 4 ; : 436-444. 74. Greene WL, Concato J, Feinstein AR. Claims of equivalence in medical research: are they supported by the evidence? Ann Intern Med. 2000; 132 9 ; : 715-722. 75. Kaul S, Diamond GA. Good enough: a primer on the analysis and interpretation of noninferiority trials. Ann Intern Med. 2006; 145 1 ; : 62-69. 76. Schistosomiasis Control Initiative. Integrated mass treatment of the neglected tropical diseases in Niger. : schisto Niger NigerNTDlaunch . Accessed August 4, 2007. 77. The Global Alliance to Eliminate Lymphatic Filariasis Web site. : filariasis . Accessed August 4, 2007. 78. Jimmy Carter and General Dr. Yakubu Gowon encourage Nigerian officials to control schistosomiasis, other diseases [press release]. February 15, 2007. The Carter Center Web site. : cartercenter news pr nigeria 021507 . Accessed August 4, 2007. 79. Hotez PJ, Bundy DAP, Beegle K, et al. Helminth infections: soil-transmitted helminth infections and schistosomiasis. In: Jamison DT, ed. Disease Control Priorities in Developing Countries. 2nd ed. New York, NY: Oxford University Press; 2006: 467-482. 80. Sachs JD. The neglected tropical diseases [abstract]. Sci Am. 2007; 296 1 ; : 33A. 81. Sachs JD. The End of Poverty: Economic Possibilities for Our Time. New York, NY: Penguin Press; 2005. 82. Sachs JD, McArthur JW. The Millennium Project: a plan for meeting the Millennium Development Goals. Lancet. 2005; 365 9456 ; : 347-353. 83. Kristof ND. Attack of the worms. New York Times. July 2, 2007. 84. Global Network for Neglected Tropical Disease Control. Alyssa Milano named founding ambassador of global network for neglected tropical disease control [press release]. : gnntdc.sabin files sabin milano announcement release 06 28 07 Accessed August 4, 2007. 85. Yamey G, Hotez P. Neglected tropical diseases. BMJ. 2007; 335 7614 ; : 269-270. 86. Global Network for Neglected Tropical Disease Control. Global network for neglected tropical disease control chairman and founder, Dr. Peter Hotez, named ambassador in Paul G. Rogers Society for Global Health [press release]. : gnntdc.sabin files documents . Published November 16, 2006. Accessibility verified September 20, 2007. 87. Hotez P, Ottesen E, Fenwick A, Molyneux D. The neglected tropical diseases: the ancient afflictions of stigma and poverty and the prospects for their control and elimination. Adv Exp Med Biol. 2006; 582: 23-33. Global Network for Neglected Tropical Disease Control. Control of NTDs. : gnntdc.sabin what control #Drug%20Donations. Accessed July 19, 2007. 89. Global Network for Neglected Tropical Diseases Web site. : gnntdc.sabin network index . Accessed September 26, 2007. 90. Hotez PJ, Molyneux DH, Fenwick A, et al. Control of neglected tropical diseases. N Engl J Med. 2007; 357 10 ; : 1018-1027. 91. Lietman TM, Neuwelt MD, Gandhi NG, Dalman CA, Benitah N. Trachoma research: it takes more than a village. Lancet. 2006; 367 9508 ; : 395. 92. Global Network for Neglected Tropical Diseases Web site. Neglected tropical diseases, malaria and HIV AIDS. : gnntdc.sabin what ntds . Accessed September 26, 2007. 93. World Health Organization. Report of the WHO informal consultation on the use of praziquantel during pregnancy and lactation and albendazole mebendazole in children under 24 months, April 8-9, 2002. : who.int wormcontrol documents en pvc 20024full . Accessibility verified September 27, 2007. 94. Savioli L, Crompton DW, Neira M. Use of anthelminthic drugs during pregnancy. J Obstet Gynecol. 2003; 188 1 ; : 5-6. 95. World Health Organization. Report of the WHO informal consultation on hookworm infection and anaemia in girls and women: Geneva December 5-6, 1994. : who.int wormcontrol documents publications en 96-1 . Accessibility verified September 20, 2007. 96. World Health Organization. Preventive chemotherapy in human helminthiasis. : whqlibdoc .who.int publications 2006 9241547103 eng . Accessed September 9, 2007. 97. Hotez P, Bethony J, Brooker S, Albonico M. Eliminating neglected diseases in Africa. Lancet. 2005; 365 9477 ; : 2089. 98. Behnke JM, Pritchard DI, Wakelin D, et al. Effect of ivermectin on infection with gastro-intestinal nematodes in Sierra Leone. J Helminthol. 1994; 68 3 ; : 187-195. 99. Albonico M, Engels D, Savioli L. Monitoring drug efficacy and early detection of drug resistance in human soil-transmitted nematodes. Int J Parasitol. 2004; 34 11 ; : 1205-1210. 100. Albonico M, Smith PG, Ercole E, et al. Rate of reinfection with intestinal nematodes after treatment of children with mebendazole or albenadazole in a highly endemic area. Trans R Soc Trop Med Hyg. 1995; 89 5 ; : 538-541. 101. Leach AJ, Shelby-James TM, Mayo M, et al. A prospective study of the impact of community-based azithromycin treatment of trachoma on carriage and resistance of Streptococcus pneumoniae. Clin Infect Dis. 1997; 24 3 ; : 356-362. 102. Sundar S, Jha TK, Thakur CP, et al. Injectable paromomycin for visceral leishmaniasis in India. N Engl J Med. 2007; 356 25 ; : 2571-2581. 103. The Carter Center Web site. : www rtercenter . Accessed August 4, 2007. 104. Barry M. The tail end of guinea worm: global eradication without a drug or a vaccine. N Engl J Med. 2007; 356 25 ; : 2561-2564. 105. Simeon DT, Grantham-McGregor SM, Callender JE, Wong MS. Treatment of Trichuris trichiura infections improves growth, spelling scores and school attendance in some children. J Nutr. 1995; 125 7 ; : 1875-1883.
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Reasonably be called different states of the system, and they are characterized by somewhat different energies. Fig. 5 a shows the total potential energy of the system as a function of time. Because of the noise, it is difficult to discern the energy levels of the two states and the crossing of the energy barrier between them. The noise level can be reduced by doing energy minimizations on the various conformations that are generated in time 22, 23 ; . The results of doing energy minimizations every picosecond on the conformations that are generated in the dynamics run are given in Fig. 5 b. It seen that the energy levels of the two states of water conformation and the barrier between them are made more clearly visible. The Mackay et al. conformation with all the water dipole projections along the channel axis aligned in the same direction has a higher potential energy than the earlier conformation. However we have no way to judge from these results as to which conformation has the higher free energy. Because our molecular dynamics simulations and those of Mackay et al. are by necessity in the picosecond regime tens of picoseconds ; , it is not clear as to what extent the different water conformations seen in the two simulations are caused by the differences in the models force fields ; or to what extent we happen to have sampled different regions in the total multidimensional space of possible water conformations.
1. Corzett M, Mazrimas J, Balhorn R. Protamine 1: protamine 2 stoichiometry in the sperm of eutherian mammals. Mol Reprod Dev 2002; 61: 519527. Cho C, Willis WD, Goulding EH, Jung-Ha H, Choi YC, Hecht NB, Eddy EM. Haploinsufficiency of protamine-1 or -2 causes infertility in mice. Nat Genet 2001; 28: 8286. Braun RE, Behringer RR, Peschon JJ, Brinster RL, Palmiter RD. Genetically haploid spermatids are phenotypically diploid. Nature 1989; 337: 373376. Caldwell KA, Handel MA. Protamine transcript sharing among postmeiotic spermatids. Proc Nat Acad Sci U S A 1991; 88: 24072411. Balhorn R, Reed S, Tanphaichitr N. Aberrant protamine 1 protamine 2 ratios in sperm of infertile human males. Experientia 1988; 44: 52 de Yebra L, Ballesca JL, Vanrell JA, Bassas L, Oliva R. Complete selective absence of protamine P2 in humans. J Biol Chem 1993; 268: 1055310557. Morris ID, Ilott S, Dixon L, Brison DR. The spectrum of DNA damage in human sperm assessed by single cell gel electrophoresis comet assay ; and its relationship to fertilization and embryo development. Hum Reprod 2002; 17: 990998. Steele EK, McClure N, Maxwell RJ, Lewis SE. A comparison of DNA damage in testicular and proximal epididymal spermatozoa in obstructive azoospermia. Mol Hum Reprod 1999; 5: 831835. Kuretake S, Kimura Y, Hoshi K, Yanagimachi R. Fertilization and development of mouse oocytes injected with isolated sperm heads. Biol Reprod 1996; 55: 789795. Chatot CL, Ziomek CA, Bavister BD, Lewis JL, Torres I. An improved culture medium supports development of random-bred 1-cell mouse embryos in vitro. J Reprod Fertil 1989; 86: 679688. Haines G, Marples B, Daniel P, Morris I. DNA damage in human and mouse spermatozoa after in vitro-irradiation assessed by the comet assay. Adv Exp Med Biol 1998; 444: 7993. Belokopytova IA, Kostyleva EI, Tomilin AN, Vorob'ev VI. Human male infertility may be due to a decrease of the protamine P2 content in sperm chromatin. Mol Reprod Dev 1993; 34: 5357. Harrouk W, Codrington A, Vinson R, Robaire B, Hales BF. Paternal exposure to cyclophosphamide induces DNA damage and alters the expression of DNA repair genes in the rat preimplantation embryo. Mutat Res 2000; 461: 229241. Ahmadi A, Ng SC. Fertilizing ability of DNA-damaged spermatozoa. J Exp Zool 1999; 284: 696704. Balhorn R. A model for the structure of chromatin in mammalian sperm. J Cell Biol 1982; 93: 298305. Ward WS, Coffey DS. DNA packaging and organization in mammalian spermatozoa: comparison with somatic cells. Biol Reprod 1991; 44: 569574. Ward WS, Kimura Y, Yanagimachi R. An intact sperm nuclear matrix 18. 19.
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Concentration of glycerol in the reaction mixture resulted in considerably more product in the first binding event for Dpo4-catalyzed incorporation of dCTP opposite O6-MeG supplemental Fig. S4 ; . Therefore, the pre-steady-state reactions were repeated using reaction buffer containing 35% glycerol v v ; . Polymerase catalysis was stopped via addition of 500 mM EDTA pH 9.0 ; . Where indicated, competitor primer template DNA 1 M 13 18mer ; was included in the right syringe as a trap for protein, thereby creating single-turnover conditions even under enzyme limiting conditions. Substrate and product DNA was separated by electrophoresis on a 20% polyacrylamide w v ; 7 urea gel. The products were then visualized using a phosphorimager and quantitated using Quantity OneTM software BioRad, Hercules, CA ; . Results obtained under single-turnover conditions were fit to the equation.
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Sterols mediate feedback inhibition of the sterol regulatory element-binding protein SREBP ; pathway by preventing movement of the SREBP cleavage-activating protein SCAP ; SREBP complex from endoplasmic reticulum ER ; to Golgi, where proteolytic cleavage of SREBPs releases the transcription factor domain that activates genes for lipid biosynthesis. Our laboratory previously used a trypsin cleavage assay to show that the conformation of SCAP is altered in vitro by addition of cholesterol to ER membranes. More recently, Insig-1 and Insig-2 were identified as ER resident proteins that bind the SCAP SREBP complex and promote its ER retention when cells are treated with sterols. Here, we use the trypsin assay to show that Insig proteins reduce the concentration of cholesterol needed in vitro to produce the conformational change in SCAP. Insig-1 and Insig-2 also enhance the conformational change in SCAP that occurs upon addition of certain cationic amphiphiles, such as chlorpromazine, trifluoperazine, and imipramine, which mimic the effect of cholesterol. The effects of cationic amphiphiles raise the possibility that SCAP may monitor specifically the composition of the cytoplasmic leaflet of the ER membrane.
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Spermatozoa were incubated for 30 min at 37 C BWW alone or supplemented with 500 M IBMX or 50 M milrinone. Intracellular cAMP was extracted with ice-cold 90% ethanol and measured by radioimmunoassay using [125I]cAMP as described previously [7]. The level of cAMP in spermatozoa incubated in BWW alone was subtracted from that obtained in spermatozoa treated with IBMX or milrinone.
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2. Moertl CG, Fleming TR, MacDonald JS, et al. Levamisole and fluorouracil for adjuvant therapy of resected colon carcinoma. N Engl J Med. 1990; 322: 352-358. NIH Consensus Conference. Adjuvant therapy for patients with colon and rectal cancer. JAMA. 1990; 264: 1444-1450. Brower M, Asbury R, Kramer Z, Pandya K, Bennett M. Adjuvant chemotherapy for colorectal cancer in the elderly: population-based experience [abstract]. Soc Clin Oncol Proc. 1993; 12: 195. Coburn MC, Pricolo VE, Soderburg CH. Factors affecting prognosis and management of carcinoma of the colon and rectum in patients more than eighty years of age. J Coll Surg. 1994; 179: 65-69. Forman WB. The role of chemotherapy and adjuvant therapy in the management of colorectal cancer. Cancer. 1994; 74: 2151-2153. Ross PJ, Popescu D, Cunningham A, et al. Adjuvant and palliative chemotherapy for colorectal cancer in patients aged 70 years or older [abstract]. Soc Clin Oncol Proc. 1998: 1096. 8. Shulman KA, Berlin JA, Harless W, et al. The effect of race and sex on physicians' recommendations for cardiac catheterization. N Engl J Med. 1999; 340: 618-625. Balducci L, Mowry K. Pharmacology and organ toxicity of chemotherapy in older patients. Oncology. 1992; 6: 62-68 and lexiva.
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Hashimoto-Gotoh, T., Tsujimura, A., Kuriyama, K., Matsuda, S., 1993. Construction and characterization of new hostvector systems for the enforcement-cloning method. Gene 137, 211 216. Kovach, M.E., Elzer, P.H., Steven Hill, D., Robertson, G.T., Farris, M.A., Martin Roop II, R., Peterson, K.M., 1995. Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene 166, 175 176. Lundblad, A., 1980. Urinary amino acid glycosides and oligosaccharides in health and disease. Scand. J. Clin. Lab. Invest., Suppl. 154, 3 11. Mahenthiralingam, E., Coenye, T., Chung, J.W., Speert, D.P., Govan, J.R., Taylor, P., Vandamme, P., 2000. Diagnostically and experimentally useful panel of strains from the Burkholderia cepacia complex. J. Clin. Microbiol. 38, 910 913. Ofek, I., Doyle, R.J., 1994. Bacterial Adhesion to Cells and Tissues. Chapman and Hall, New York. Ralph, P., Nakoinz, I., 1977. Direct toxic effects of immunopotentiators on monocytic, myelomonocytic, and histiocytic or macrophage tumor cells in culture. Cancer Res. 37, 546 550. Schulz, B.L., Oxley, D., Packer, N.H., Karlsson, N.G., 2002. Identification of two highly sialylated human tear-fluid DMBT1 isoforms: the major high-molecular-mass glycoproteins in human tears. Biochem. J. 366, 511 520 and librium.
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